Figure 5.

PRSS23 knockdown decreased FGF2 expression and secretion in GC. (A) RNA-seq studies were conducted in GC cells transfected with siRNAs targeting PRSS23. The most significantly altered genes upon PRSS23 knockdown were shown in the heatmap. (B, C) The transcripts abundance of PRSS23 and FGF2 in PRSS23-depleted GC cells was detected by RNA-seq. The normalized expression (FPKM value) of PRSS23 and FGF2 were shown in the plot. (D) The knockdown efficiency of PRSS23 in GC cell lines was examined by qRT-PCR assay. (E) The effect of PRSS23 knockdown on FGF2 expression in GC cell lines were examined by qRT-PCR assay. (F) PRSS23 and FGF2 were highly co-expressed in GC. (G) The effect of PRSS23 knockdown on FGF2 protein level in GC cell lines were examined by western blotting assay. (H, I) ELISA assay showed that PRSS23 knockdown significantly decreased secreted FGF2 level in GC cell lines. **, P < 0.01.