Skip to main content
. 2022 Sep 15;13:884037. doi: 10.3389/fgene.2022.884037

TABLE 1.

Specific primers for RT-QPCR.

Name (ID) Type Sequence (5′-3′) Product size (Bp)
Circ_0001452 Forward TAG​CAG​CAA​CCT​GGT​CTC​TTT 119
Reverse ATC​AGC​AAG​GCA​GAG​AGG​TG
Circ_0001453 Forward GCA​ACC​TGG​TCT​CTT​TTC​ACG 104
Reverse CCC​GAA​AGT​GAG​GCA​AGG​AC
Circ_0001454 Forward TGA​CTC​TGG​GCT​CTC​AGG​AA 216
Reverse ATC​AGC​AAG​GCA​GAG​AGG​TG
Circ_0000503 Forward CAG​TCG​TGG​AAT​CTG​TGG​GT 242
Reverse TCA​TCC​AGC​TCT​GCT​CCA​TC
MiR-466i-3p (MI0006282) Stem-loop GTC​GTA​TCC​AGT​GCA​GGG​TCC​GAG 66
GTA​TTC​GCA​CTG​GAT​ACG​ACT​AGT​GT
Forward GGC​ATA​CAC​ACA​CAC​ATA​CAC​AC 60
Reverse AGT​GCA​GGG​TCC​GAG​GTA​TT
MiR-669c-3p (MI0004673) Stem-loop GTC​GTA​TCC​AGT​GCA​GGG​TCC​GAG 66
GTA​TTC​GCA​CTG​GAT​ACG​ACT​TTA​CT
Forward CGC​TAC​ACA​CAC​ACA​CAC​AAG​TAA​A 60
Reverse AGT​GCA​GGG​TCC​GAG​GTA​TT
AMPK-α1 (105787) Forward GCC​TTG​AAA​GAA​GTG​TGT​GAG​AAG​TTC 85
Reverse GTG​GGT​CCT​GGT​GGT​TTC​TGT​TG
SREBP1 (20787) Forward GCT​ACC​GGT​CTT​CTA​TCA​ATG​A 91
Reverse CGC​AAG​ACA​GCA​GAT​TTA​TTC​A
FASN (14104) Forward TAA​AGC​ATG​ACC​TCG​TGA​TGA​A 230
Reverse GAA​GTT​CAG​TGA​GGC​GTA​GTA​G
GAPDH (14433) Forward GGT​TGT​CTC​CTG​CGA​CTT​CA 183
Reverse TGG​TCC​AGG​GTT​TCT​TAC​TCC
U6 (19862) Forward CTC​GCT​TCG​GCA​GCA​CAT​ATA​C 82
Reverse ATT​TGC​GTG​TCA​TCC​TTG​CG

AMPK-α1, Adenosine 5’-monophosphate-activated protein kinase α1; FASN, fatty acid synthase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; SREBP1, sterol regulatory element-binding protein 1.