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. 2022 Sep 15;13:979610. doi: 10.3389/fmicb.2022.979610

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Copyright © 2022 Cafora, Poerio, Forti, Loberto, Pin, Bassi, Aureli, Briani, Pistocchi and Fraziano.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PS/PA liposomes and CKΦ administration does not elicit toxic effects on human CF cells. (A) Cell viability, evaluated by MTT assay, in immortalized human bronchial epithelial cells CuFi-1 expressing F508del CFTR control, treated with phosphatidylserine/phosphatidic acid (PS/PA) liposome, CKΦ or combination of PS/PA liposome-CKΦ for 48 h. (B) Cell viability, evaluated by MTT assay, in THP-1 activated macrophage-like cells treated or not with the CFTR inhibitor. Effects of single or combined PS/PA liposome and CKΦ-administration were evaluated after 48 h of treatment. Unpaired Student’s t test: *p < 0.05; ns, not significant. Results are presented as mean ± SEM.