Fig. 7.

RNA interference and pathway analysis of TLN1. A HL60 and THP-1 cells were transfected with siRNA, the level of TLN1 was evaluated by qRT-PCR. B Wright–Giemsa staining of control and TLN1-silenced cells. The flow cytometric analysis was carried out to analysis the cell cycle distribution (C-D). E The proliferation of THP-1 and HL60 was examined by cell counts. F The expression levels of apoptosis-related proteins were evaluated by Western blot in control and TLN1-silenced cells. G The expression levels of Talin1/p-AKT/CREB proteins were evaluated by Western blot in control and TLN1-silenced cells. Data were shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001