TABLE 1.
Characteristics of plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pBluescript | ColE1 lacZ bla | Stratagene |
| pG209 | 1 kb of the YlGPI7 gene in pGEM-T easy | This study |
| pG209URA | YlURA3 gene in pG209 | This study |
| pG354/CRE | 1.3 kb of fil354 locus after mTnYl1 excision in pGEM-T easy | This study |
| pG354/CRE/URA | YlURA3 gene in pG354/CRE | This study |
| pGEM-T easy | oriF1 lacZ bla | Promega Corp., Madison, Wis. |
| pINA156 | YlURA3 in pUC13 | This laboratory collection |
| pINA240 | YlLEU2 ARS68 in pBR322 | This laboratory collection |
| pINA444 | YlLEU2 ARS68 in pBR322 | This laboratory collection |
| pINA1053 | YlLEU2 ARS68 lacZ | This laboratory collection |
| pINA1269 | YlLEU2 ARS68 PH4D promoter XPR2 terminator | This laboratory collection |
| pICT246 | YlLEU2 ARS18; HOY1 with its own promoter | 66 |
| pKSURA | YlURA3 in pBluescript | 70 |
| pMGF1 | YlLEU2, YlPHD1 in pBluescript | A. Dominguez laboratory collection |
| pMR1 | 2.6 kb of the fil316 disruption cassette in pGEM-T easy | This study |
| pMR2 | 0.9-kb fragment of YlRAS2 gene containing I-SceI site in pGEM-T easy | This study |
| pMR3 | 2.1-kb fragment containing YlURA3 in pMR1 at I-SceI site | This study |
| pMR4 | YlPHD1 from p1.69 in pINA240 | This study |
| pMR5 | 2.5 kb of the fil23 disruption cassette in pGEM-T easy | This study |
| pNEB193 | ori F1 lacZ bla | New England Biolabs, Inc., Beverly, Mass. |
| pRRQ1 | 0.8-kb fragment containing promoter from p1269 in pINA1053 | This study |
| pRRQ2 | 1.4-kb fragment containing CRE from pSH47 in pRRQ1 | This study |
| pRRQ10 | HOY1 from pJCT246 in pINA444 | This study |
| pSH47 | YlURA3 ARSH4 CEN6 CRE under GAL1 promoter and CYC1 terminator | 26 |
| pUCURA | 1.2-kb fragment of YlURA3 from pINA156 in pNEB193 | This study |
| p1.69 | HOY1 gene in pBluescript | A. Dominiguez laboratory collection |