TABLE 2.
Oligonucleotide sequences of primers used in this study
| Primer | Sequence (5′→3′)a |
|---|---|
| 3161up | AATCTCACGTCCGAATGTCC |
| 3161dw | AATAGCCCTAGGTCTACCACTCGTTTGGTCCC |
| 3162up | AATAGCATCGATAGATCTCATATCGCTTGGCG |
| 3162dw | GATGAACGGCATGAACATGG |
| 3451upL | GTGTTACCGGTATAAGTCGCACG |
| 3451dwSceI | CATTACCCTGTTATCCCTAATAGCATCAAGGGTGGTACCAAGTG |
| 3452upSceI | CTAGGGATAACAGGGTAATTGTTGACCGGTGGTCTGAGTCGACC |
| 3452dwL | TTTGTGTTGTGGAGCTGTGCCATCG |
| 209DEL1 | GACATAGTTACGTGGCTGTCCAAGG |
| 209DEL2 | GATGCTTCTCTGAGGAAAAAGCAAG |
| 209DEL3 | CCCCTCATATTCTCATCTCAATCCC |
| 246DEL1 | CGATAGTGACGCTGACATTCGTCTG |
| 246DEL2 | GGGTCTGAGATGGAACGAAATAAGC |
| URA3-A | CCACCAAAATGCCCTCC |
| URA3-B | TGGTAGTGCAGTGGTGG |
| 354DEL1 | CACCCCACTACACAAACTATGCAAC |
| 354DEL2 | CATCTGATGCTGGCAATCGACGAAC |
| mtn1 | GGAGTTATCCGAAGCGATAGCG |
| mtn6 | GCGGGCTATCCCTATGAC |
| juan1 | GGCGTAGGTGAGTCGTC |
| juan2 | CTCAAGCTCGTGGCAGCC |
| Adapter XbaI | CTAGAGAACCCCTTCG |
| Adapter BglII | TCTTGGGGAAGCCTAG |
a
Underlined segments represent restriction sites used for cloning.