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Pneumolysin expression and release by wild-type and autolysin-deficient pneumococci. WU2 and SM121.10 (WU2 lytA mutant) were grown at 37°C in THY. Samples were withdrawn at different times, and the OD were determined (right y axis). The pneumolysin titers for cell pellets and supernatants were determined (left y axis). The pneumolysin titers for the first two points were below the detection limits of the assay (titer = 3) and were not plotted.
