FIG. 6.

Analyses of total proteins in the cytoplasm and supernatant during growth. WU2 was grown in THY at 37°C. Samples were withdrawn at various times. The cells were pelleted and lysed. The proteins present in the lysed pellets (cytoplasmic) and the culture supernatant (secreted) were precipitated with 10% TCA. The pellet (a) and supernatant (b) proteins were separated on a 10% polyacrylamide gel and stained with Coomassie brilliant blue R-250. THY alone was run as a control for the supernatant samples (b).