Figure 2.

Metaphase chromosomes of C. ciliaris (2n = 4x + 3 = 39) after FISH (red fluorescing, (i) and immunostaining with anti-5-methylcytosine (green fluorescing, (ii) and as an overlap image (iii). (A) The “universal” Ty1-copia probe shows concentrated signal around the centromeres of all chromosomes, while 5-mC signal is not only seen overlapping them but also further along the chromosome arms. 5-mC strength is variable between the chromosomes with most showing a small gap at the centromeres. (B) The “universal” Ty3-gypsy probe shows a dispersed signal over the chromosome arms, but is absent from the most distal parts. 5-mC signal covers most Ty3-gypsy signals but is weak or absent in some cases (arrows). Scale bar = 10 μm.