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. 2022 Mar 31;107(10):2395–2407. doi: 10.3324/haematol.2021.280169

Figure 2.

Figure 2.

Anti-tumor function of BCMA-CAR-T cells. (A) OKT3-activated human primary B-cell maturation antigen (PBMC) were transduced with a retroviral vector encoding either ICBB-, H828-, H8BB-CAR, or truncated CD34 (CD34t control). Transduced T cells were co-cultured with different tumor lines as indicated. Interferon γ (IFNγ) secreted in the co-culture supernatant was measured by enzyme-linked immunosorbant assay (ELISA). These results are presented as mean+ standard error of the mean (SEM) (n=4, with 3 different donors). The difference between H8BB and H828 was found statistically significant (P=0.00098; calculated using a paired Student’s t-test). (B and C) Similarly, these cells were co-cultured with the indicated target T cells and TNFα (B) and IL-2 (C) concentrations secreted in the culture supernatant were determined by ELISA. These results are presented as mean+SEM (n=3, with 3 different donors). P=0.01 for IL-2 and P=3.7x10-5 for TNFα; by paired Student’s t-test). BCMA: B-cell maturation antigen; CAR: chimeric antigen receptor.