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DEAE-Sephacel anion-exchange column chromatography profiles of OPGs from E. chrysanthemi grown in LOS medium (A) or LOS medium with 0.3 M NaCl (B). Ionic strength was increased by steps of 0.05 M NaCl at the fractions indicated by the arrows. Fractions (4 ml) were collected, and total carbohydrate concentrations were determined by the anthrone method (see Materials and Methods). The amount of glucans in each fraction is expressed as a percentage of the total amount loaded on the column.
