TABLE 3.
Methylation analysis of HPAEC fractions 3, 4, 5, and 6
| Fraction no. | % Methylated sugara (molar ratio)
|
|||
|---|---|---|---|---|
| Terminal Glc | 2-Linked Glc | 6-Linked Glc | 2,6-Linked Glc | |
| 3 | 35 (2.4) | 32 (2.2) | 7 (0.5) | 26 (1.9) |
| 4 | 25 (1.8) | 55 (3.8) | 3 (0.2) | 17 (1.2) |
| 5 | 36 (2.9) | 27 (2.2) | 8 (0.6) | 29 (2.3) |
| 6 | 31 (2.5) | 44 (3.5) | 5 (0.4) | 20 (1.6) |
a
As methyl-O-acetylglucosides after methanolysis and peracetylation as described in Materials and Methods. The molar ratio is based on the degree of polymerization observed in the MALDI spectrum for each fraction.