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. 2022 Sep 15;4:969203. doi: 10.3389/fmedt.2022.969203

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© 2022 Jonguitud-Borrego, Malcı, Anand, Baluku, Webb, Liang, Barba-Ostria, Guaman, Hui and Rios-Solis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(created with BioRender.com). Schematic illustration of three different automated diagnostic workflows from patient samples and their corresponding required equipment (A) RT-PCR diagnostic workflow (B) LAMP diagnostic workflow (C) CRISPR-Cas13a nucleic acid detection workflow Equipment used for each workflow is also shown in the figure. A qPCR device is a necessity for RT-qPCR work while the plate reader is used for CRISPR-Cas and LAMP workflows to detect absorbance change. Figures modified and adapted from Crone et al. (2).