Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 15;4:969203. doi: 10.3389/fmedt.2022.969203

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Jonguitud-Borrego, Malcı, Anand, Baluku, Webb, Liang, Barba-Ostria, Guaman, Hui and Rios-Solis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

(created with BioRender.com). Different protocols using the open-source OT-2 automated platform for COVID-19 testing (A) automated workflow Designed at CBD. An initial run preparation was performed using open-source Python coding. Initial sample setup, sample preparation, and plate filling and qPCR mix preparation were performed by OT-2 robots. RNA extraction was processed by KingFisher Flex and RT- qPCR was run by ABI 7500. Analysis results were exported as a user-friendly R file. Figure adapted from Villanueva-Cañas et al. (33) (B) the layout and workflow of a semi-automated CONTAIN lab. Three separate sections were used for plating samples, RNA extraction and RT-qPCR respectively. OT-2 robots were used for the RNA extraction process and qPCR mix preparation. Station C which is used for the RT-qPCR run also contained two subsections to separate OT-2 and qPCR devices. Image taken from OpenCell.bio, Walker et al. (35).