TABLE 2.
Expression of pel and out genes in the presence of an opgG mutation in E. chrysanthemia
| Enzyme | Fusion | Inducer | Sp act (nmol of product liberated/min/mg (dry wt) ± SD
|
|
|---|---|---|---|---|
| opgG+ | opgG | |||
| Pectate lyase | − | 0.05 ± 0.02 | 0.02 ± 0.01 | |
| + | 19.7 ± 3.2 | 8.4 ± 2.1 | ||
| β-Galactosidase | pelE-lacZ | − | 7 ± 3 | 5 ± 2 |
| + | 132 ± 18 | 55 ± 9 | ||
| pelC-lacZ | − | 5 ± 2 | 2 ± 2 | |
| + | 863 ± 82 | 432 ± 25 | ||
| outC-lacZ | − | 87 ± 5 | 58 ± 6 | |
| + | 340 ± 20 | 156 ± 32 | ||
a
Cultures were grown in minimal medium to late log phase in the presence of either glycerol (−) or the inducing compounds polygalacturonate and galacturonate (+). The results reported are averages of at least three independent experiments ± standard deviation. Pectate lyase specific activity reflects the expression of the five major pel genes (pelA to pelE). β-Galactosidase specific activity reflects the expression of the corresponding lacZ fusion.