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. 2022 Sep 15;9:943099. doi: 10.3389/fvets.2022.943099

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Copyright © 2022 Yang, Peng, Song, Zhang, Fan, Chen, Hua, Pei, Tang, Chen and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Detection limit and standard curves of the triplex real-time PCR method developed in this study. (A) Detection limit for B646L; (B) Detection limit for MGF_360-14L; (C) Detection limit for CD2v; (D) Plasmid DNA standard curve for B646L, y = −3.903x + 47.88, R² = 0.9984; (E) Plasmid DNA standard curve for MGF_360-14L, y = −4.037x + 48.13, R² = 0.9991; (F) Plasmid DNA standard curve for CD2v gene, y = −3.969x + 44.90, R² = 0.9992.