Table 2.

Validation of the Detection repeatability of the developed triplex real-time PCR method.

Genes	DNA (copies/μL)	Rounds of testing	Ct meana	Ct Standard deviation	C.V.a	C.V. between groups
B646L	7.89 × 102	1	33.34	0.24	0.73%	0.74%
		2	32.99	0.26	0.79%
		3	33.47	0.42	1.25%
	7.89 × 103	1	29.53	0.11	0.37%	0.49%
		2	29.33	0.16	0.54%
		3	29.60	0.21	0.70%
	7.89 × 104	1	26.68	0.09	0.33%	0.17%
		2	26.59	0.08	0.29%
		3	26.63	0.05	0.19%
	7.89 × 105	1	23.14	0.12	0.54%	0.19%
		2	23.16	0.09	0.40%
		3	23.07	0.02	0.11%
	7.89 × 106	1	19.15	0.13	0.67%	0.54%
		2	18.95	0.15	0.77%
		3	19.02	0.09	0.46%
MGF_360-14L	4.70 × 102	1	35.29	0.66	1.86%	1.35%
		2	34.41	1.40	4.06%
		3	34.58	0.20	0.56%
	4.70 × 103	1	30.53	0.06	0.18%	0.36%
		2	30.62	0.05	0.16%
		3	30.41	0.09	0.30%
	4.70 × 104	1	26.06	0.19	0.71%	0.28%
		2	25.96	0.23	0.90%
		3	26.10	0.06	0.24%
	4.70 × 105	1	22.46	0.04	0.16%	0.56%
		2	22.49	0.09	0.41%
		3	22.69	0.18	0.78%
	4.70 × 106	1	18.49	0.05	0.28%	0.14%
		2	18.44	0.13	0.72%
		3	18.48	0.05	0.28%
CD2v	8.21 × 102	1	32.18	0.16	0.51%	0.29%
		2	32.03	0.20	0.63%
		3	32.01	0.36	1.11%
	8.21 × 103	1	29.40	0.07	0.25%	0.31%
		2	29.39	0.05	0.17%
		3	29.55	0.11	0.36%
	8.21 × 104	1	26.48	0.17	0.64%	0.23%
		2	26.43	0.30	1.15%
		3	26.36	0.42	1.61%
	8.21 × 105	1	23.78	0.06	0.27%	0.01%
		2	23.78	0.04	0.19%
		3	23.78	0.06	0.25%
	8.21 × 106	1	19.76	0.07	0.35%	0.18%
		2	19.69	0.02	0.10%
		3	19.75	0.10	0.53%

^aCt, cycle threshold; C.V., coefficient of variation.