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. 2022 Sep 28;28(36):5250–5264. doi: 10.3748/wjg.v28.i36.5250

Table 2.

Research progress on the clinical utility of transforming growth factor-beta 1 as a prognostic marker against hepatocellular carcinoma

Ref.
Sample size HCC/control
Sample and assay type
TGF-β1 level
Survival rate (%), (patients with higher TGF-β1 vs patients with lower TGF-β1)
Outcome of the study
[75] 571/551 Plasma Control: 3.58 ± 0.17 log10 pg/mL 1 yr survival (47 vs 60) 3 yr survival (28 vs 36) P < 0.05 Plasma TGF-β1 levels showed a positive correlation with tumor size, invasion and extrahepatic metastasis and inversely correlated with survival rates in HCC patients
ELISA Cirrhotic: 3.20 ± 0.37 log10 pg/mL
HCC: 3.83 ± 0.31 log10 pg/mL
[83] 126 Tumor tissue 84% samples (106/126) showed high intra-tumoral TGF-β1 expression 5 yr survival (8.5 vs 45.6) TGF-β1 and FGFR4 were positively correlated in HCC tumor tissues and showed a significant association with shorter survival rates in patients
Immunohistochemistry 64.3% samples (81/126) showed high peri-tumoral TGF-β1 expression
[80] 84/20 Tumor tissue TGF-β1 overexpression found in 59.5% samples (50/84) than that of normal liver tissue 1 yr survival (28 vs 79.4) 5 yr survival (12 vs 62.6) TGF-β1 expression was dominant, whereas ELF expression was suppressed in HCC tissues
Immunohistochemistry Patients with high TGF-β1 and lower ELF expression are associated with poor overall survival and post-operative disease free survival compared with low TGF-β1 and high ELF group
[76] 184/30 Plasma and tumor tissue Elevated plasma TGF-β1 level 2 yr survival ( 51 vs 77) 3 yr survival (4 vs 68), P < 0.05 Higher TGF-β1 expression in tumor tissues triggers Treg cells mediated immunosuppression in tumor microenvironment and contribute to poor prognosis in HCC
ELISA and immunohistochemistry TGF-β1 was strongly stained in tumor tissue
[84] 40 Serum Before RFA: 63.22 ± 23.61 ng/mL After RFA: 56.33 ± 24.24 ng/mL NA Radiofrequency ablation lowered TGF-β1 and AFP L3% expression in HCC patients
ELISA Low TGF-β1 and AFP L3% levels were observed in the no recurrence group, suggesting its potential as prognostic markers for HCC

ELISA: Enzyme-linked immunosorbent assay; HCC: Hepatocellular carcinoma; TGF-β: Transforming growth factor-beta; AFP: Alpha feto protein; ELF: Embryonic liver fodrin; FGFR: Fibroblast growth factor receptor 4.