TABLE 3.

Changes in the H box region which relieved the dominant-negative effect of GST-Bc

GST-Bc variant	No. of isolates	Presence of uhpT-lacZ expression in strain RK1309 (uhp+)			Uhp phenotypeb
		−Glc6P	+Glc6P	+Glc6P and IPTGa
GST		−c	+	+	I
GST-Bc (293–500)		−	−	−	DN
L293P/D356A	1	+	+	+	C
I316T	1	−	+	+	I
I316N/S351L	1	−	+	+	I
T321S/T325P	1	−	+	+	I
R324C	3	+	+	+	C
A327E/V340G	1	−	+	+	I
V330D	1	−	+	+	I
G344R	1	−	+	+	I
L352P	2	−	+	+	I
Y355C	1	+	+	+	C
V358E	1	−	+	+	I

^a25 μM IPTG. 

^bI, inducible; DN, dominant negative; C, constitutive. 

^c−, no uhpT-lacZ expression; +, uhpT-lacZ expression was present.