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. 2022 Sep 21;61(5):136. doi: 10.3892/ijo.2022.5426

Figure 1.

Figure 1

MDR HCT-116 cells are resistant to cisplatin-induced apoptosis. (A) The protein expression levels of the ABCB1 and GSTO1 proteins in WT and MDR HCT-116 cells were analyzed by western blotting and quantified using ImageJ software. Unpaired Student's t-test was used for statistical analysis. WT and MDR cancer cells were treated with 50 µM cisplatin for 12 h, and cell apoptosis was analyzed by (B) LDH assay and (C) ApoStrand™ apoptosis assay; one-way ANOVA was used for statistical analysis. (D) Nuclear damage was analyzed by Hoechst 33342 staining (×200 magnification). (E) Western blotting of apoptosis markers. (F) Mitochondrial and nuclear proteins were analyzed by western blotting to determine the expression of AIF and c-Jun proteins. *P<0.05; ***P<0.001; ****P<0.0001. AIF, apoptosis-inducing factor; cas, caspase; cis, cisplatin; GSTO1, glutathione-S-transferase Ω-1; HSP, heat-shock protein; LDH, lactate dehydrogenase; MDR, multidrug resistant; MDR1, multidrug-resistance protein 1; ns, not significant; PARP, poly(ADP-ribose) polymerase 1; veh, vehicle; WT, wild-type.