Figure 4.

: STUB1 improves the imbalance of Th17/Treg cells in AHR-dependent manner. (A) Ubiquitination of AHR was increased in RA patients compared with healthy controls. Purified CD4+T cells from peripheral blood of RA patients (n = 4) and healthy controls (n = 4). AHR ubiquitination was detected with the indicated antibody. Densitometry was performed and quantitation of ubiquitinated AHR was normalized to total AHR from lysates. (B, C) Compared effect of STUB1 on Th17/Treg cells with that of FICZ. The proportion of Th17 (CD4+IL-17+) and Treg (CD25+Foxp3+) cells was detected by flow cytometry. Percentages of Th17 cells and Treg cells are shown in the bar. ** P < .01 vs. control groups. NS, no significant (Student’s t-test). Data are pooled from three independent experiments. Error bars show mean ± SEM.