Figure 3.

Kinetics of anterograde to retrograde transport conversion of IFT trains

(A and B) Tomographic slice through ciliary tip showing an anterograde train in the process of disassembly (scale bar, 200 nm). Highlighted rectangular region is magnified in (B), showing loss of structural cohesion of the train localized at the end of the B-tubule (scale bar, 100 nm). The structured part of the train is colored in blue, the disordered part of the train in violet, the microtubule in green, and the ciliary coat in yellow. See also Video S4.

(C) Retrograde train appearance frequency at physical blockage. Transport recovers to normal steady-state level with a half-time of 2.6 ± 1.7 s (first-order response fit in blue, uncertainty bounds in light gray).

(D) Retrograde train appearance frequency at the ciliary tip after releasing the physical blockage. Steady-state retrograde transport recovers with a half-time of 0.77 ± 0.74 s (fit same as C; error bars in C and D represent SEM).

(E) Box plot of train recovery half-time at the wedge and at the ciliary tip after release.

Data at the wedge (C and E) from 895 trains in 23 experiments on 12 individual cells.

Data at the tip (D and E) from 580 individual trains in 18 experiments on 10 individual cells.

See also Figure S2.