Figure 4.

Dynamics of the IFT motors kinesin-II and dynein during anterograde to retrograde conversion

(A) Denoised kymograms of dual-labeled cilia show distinct behavior between dynein (labeled with D1bLIC-mCherry) and kinesin-II (labeled with KAP-GFP), both in the undisturbed flagellum and when blocked by a wedge. Inserts illustrate regions highlighted in (B) and (C).

(B) Background-subtracted detail at the ciliary tip. Positions of some prominent retrograde trains are marked with white arrows, with the same positions shown in the KAP-GFP detail for reference.

(C) Background-subtracted detail at the wedge. White arrows denote the location of visible retrograde trains, the positions of which are duplicated in the KAP-GFP detail for reference.

See also Figure S3.