REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
anti-IFT46	Rosenbaum lab	17600
anti-GFP	Roche	11814460001; RRID:AB_390913
Deposited data
TIRF Microscopy Timeseries	This study	https://doi.org/10.5281/zenodo.6787231
Chemicals, peptides, and recombinant proteins
PDMS	Dow Corning	Sylgard 184
Lithium Chloride	Sigma-Aldrich	203637
Tris Base	Merck	93362
Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid	Sigma	E4378
NH4Cl	Sigma	A9434
MgSO4	Unknown	N/A
KCl	Sigma	1.04936
K2HPO4	Sigma	P8281
KH2PO4	Sigma	P0662
Hutner's Trace Elements	Chlamydomonas Resource Center	N/A
Experimental models: Organisms/strains
Chlamydomonas: ift46-1::NIT	Chlamydomonas Resource Center	CC-4375
Chlamydomonas: pf2	Chlamydomonas Resource Center	CC-1025
Chlamydomonas: KAP-GFP D1bLIC-crCherry	Chlamydomonas Resource Center	CC5502
Chlamydomonas: mat3-4	Chlamydomonas Resource Center	CC3994
Recombinant DNA
IFT46-mNeonGreen	This work	N/A
PF2-GFP	Porter lab	N/A
pSI103	Chlamydomonas Resource Center	N/A
pE345	Unknown	N/A
Software and algorithms
FRAP analysis script	This work	makeFrapPlots.py
Python 3	Python Software Foundation	https://www.python.org
Geneious	Biomatters	https://www.geneious.com
Train analysis script	This work	makeDirectionalMasks.py
iQ3 imaging software	Andor	https://andor.oxinst.com/products/iq-live-cell-imaging-software/
Fiji	Fiji	https://imagej.net/software/fiji/
IMOD	Mastonarde group	https://bio3d.colorado.edu/imod/
CryoCare	Jug Lab	https://github.com/juglab/cryoCARE_T2T
CSBDdeep Noise2Void	Fiji	https://csbdeep.bioimagecomputing.com/
SerialEM	Mastonarde group	https://bio3d.colorado.edu/SerialEM/
MotionCor2	UCSF	https://emcore.ucsf.edu/ucsf-software
ICON	Sun Lab	http://www.ibp.cas.cn/feilab/research/md/202111/t20211115_6256123.html
Other
Glass Capillaries	Sutter instru-ments	BF100–50–7.5
Glass slides	Marienfeld	1000000
Holey Carbon Grids	Quantifoil	Q350AR-35