Fig 4. OsRMC-knockdown rice plants show reduced resistance to M. oryzae.
(A) Rice leaves of OsRMC-knockdown (OsRMC-kd) and wild-type (Moukoto) control (Con) lines 4 days after inoculation of M. oryzae (Ken53-33, 3.0 X 105 conidia). The M. oryzae infection test was conducted using T1 OsRMC-kd and wild-type control progenies (n = 10) derived from selfing of T0 heterozygous OsRMC-kd lines. (B) M. oryzae infection is enhanced in OsRMC-kd rice. The amount of M. oryzae fungal mass in rice leaf was monitored by quantifying the ratio of M. oryzae genomic DNA to rice genomic DNA, determined by qPCR of the respective actin genes. The average ΔΔCt value in wild-type control lines was defined as the unit for the ratio. Data are means ± SD of 10 independent determinations. Single and double asterisks indicate a significant difference at P < 0.05 and P < 0.01, respectively, according to Student’s t-test.