Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 29;18(9):e1010792. doi: 10.1371/journal.ppat.1010792

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 Takeda et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PMC Copyright notice

Fig 9 — (A) OsRMC, LOC_Os04g25650.1, and their chimeric proteins were produced in N. benthamiana and assayed for MoCel10A (CBM1: top) and mannose (bottom) binding. Simplified schemes of OsRMC (235 amino acids; red) and LOC_Os04g25650.1 (251 amino acids; green) as well as two newly generated chimeric proteins, Chimera1 (244 amino acids, comprising 97 aa [24–120] of OsRMC and 147 aa [135–281] of LOC_Os04g25650.1) and Chimera2 (242 amino acids, comprising 104 aa [31–134] of LOC_Os04g25650.1 and 138 aa [121–258] of OsRMC) are shown. Flag-tagged proteins were assayed for binding to MoCel10A-His and to mannose. Unbound and bound fractions were subjected to immunoblot analysis with an anti-Flag antibody. (B) Binding assay of single DUF26 domains (DUF26-A and DUF26-B) of OsRMC to MoCel10A (CBM1: top) and mannose (bottom).