TABLE 1.

Purification of citrate synthase from S. coelicolor^a

FPLC column fraction	Vol (ml)	Total activity (U)	Total protein (mg)	Sp act (U/mg)	Yield (%)
Crude extract	150	945	3,150	0.3	100
DEAE-Sepharose	100	294	490	0.6	31
Q-Sepharose	25	213	305	0.7	22.5
MonoQ	30	149	18.6	8	15.7
Matrex gel red A	15	134	6.4	21	14.2

^aThe primary citrate synthase detected in protein extracts of S. coelicolor J1501 grown in YEME was purified by FPLC.