TABLE 2.
Strain | Concn (mM) of:
|
|||||
---|---|---|---|---|---|---|
Pyruvate | Succinate | Fumarate | Malate | Citrate | Acetate | |
None (R2YE alone) | 0.01 | 0.5 | <0.1 | <0.1 | 0.0 | 1.7 |
MT1110 | 0.6 | 0.2 | 0.4 | <0.1 | <0.1 | 1.0 |
bldA mutant | 2.6 | 1.8 | 0.2 | <0.1 | <0.1 | 1.7 |
bldB mutant | 2.6 | 2.5 | 0.2 | <0.1 | <0.1 | 1.5 |
bldG mutant | 2.6 | 2.6 | 0.2 | <0.1 | <0.1 | 0.9 |
bldH mutant | 2.6 | 2.2 | 0.1 | <0.1 | <0.1 | 2.3 |
bldJ mutant | 2.6 | 2.3 | 0.4 | <0.1 | <0.1 | 1.3 |
Extracts were prepared from 48-h cultures grown on R2YE and analyzed for organic acid concentrations as described in Materials and Methods.