FIG. 4.

Colonial phenotypes of TCA cycle mutants. MT1110 (wild type [wt]), BZ2 (citA), BZ4 (acoA) and BZ5 (citA acoA) were streaked out on R2YE or MR5 containing 2% glucose (MR5 glucose) or mannitol (MR5 mannitol). Plates were incubated for 5 days at 30°C and photographed. Colonies had not undergone major changes in morphology upon reexamination 5 days later, with one significant exception. BZ5 colonies had continued to grow and produced gray spores between 5 and 10 days of incubation on MR5 mannitol (photographed and presented as a composite of the right side of the plate).