FIG. 7.
Suppression of defective bud-hypha transitions and filamentous growth in the cap1/cap1 mutant by exogenous cAMP or its derivative, dbcAMP. (A) The wild-type CAP1/CAP1 strain, UnoPP-1, and the cap1/cap1 mutant strain, CAC1-1A, were grown in SLAD medium with or without 10 mM cAMP or dbcAMP for 5 days at 37°C. Bars, 1 mm. (B) Bud-hypha transitions were induced at cell concentrations of 106 cells/ml in prewarmed M199+serum with or without 10 mM dbcAMP for 13 h (first [UnoPP-1] and second [CAC1-1A] columns, 20× objective; third [CAC1-1A] column, 40× objective). Bars, 30 μm.