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Refractive Indices of Commonly Encountered Materials
| Material | Refractive index |
|---|---|
| Water | 1.33 |
| Air | 1.0003 |
| Glass | 1.517 |
| Coverglass | 1.523 |
| Immersion oil | 1.516 |
| Cell cytosol | 1.38 |
| Mount | Variablea |
Mounting media for fluorescence microscopy can vary substantially. To the author's knowledge, none of the standard commercially available mounts are suitable for fixed-sample TIRFM.
