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. 2022 Sep 20;119(39):e2117988119. doi: 10.1073/pnas.2117988119

Fig. 7.

Fig. 7.

TGFB2-AS1 reverses breast cancer cell stemness. (A) GSEA of microarray profiles from MDA-231 with TGFB2-AS1 knockdown matching with stem cell activated gene set. (B) Heat-map representation of up-regulated genes relating mammalian embryonic stem cell pluripotency from IPA pathway analysis in TGFB2-AS1 knockdown group and control group. The orange color scales represent up-regulated genes, and the blue color scales represent down-regulated genes. (C) Schematic view of SMARCA4 occupancy on locus of SOX2 from ChIP-seq in MDA-231 cells with TGFB2-AS1 knockdown. Blue indicates siNC group and pink indicates siB2-AS1 group. H3K4Me3 and H3K27Ac histone mark were displayed on seven cell lines from ENCODE, on the layered H3K4Me3 and the layered H3K27Ac tracks respectively. Each color represents one cell line. (D) ChIP-qPCR shows the binding efficiency of SMARCA4 to the SOX2 using primer in MDA-231 cells with TGFB2-AS1 knockdown. The red arrow indicates the target fragment. (E and F) ChIP-qPCR shows the H3K4me3 and H3K27Ac efficient occupancy on the SOX2 promoter region in MDA-231 cells with TGFB2-AS1 knockdown. The mRNA and protein expression of SOX2 and NANOG in MDA-231 cells after transfected with siAS1 in (G) LM2 cells overexpressed TGFB2-AS1 in (H) and in MDA-231 cells and LM2 cells (I). Error bars represent mean ± SD (n = 3). Statistical significance was assessed using 2-tailed Student’s t test. *P < 0.05. **P < 0.01. ***P < 0.001.