Fig. 2.

Inactivating ywlG suppresses the cold sensitivity of a Δhpf-null mutant in a medium-dependent manner. Complementation with plasmid-encoded ywlG and hpf confirmed the YwlG-mediated suppression and HPF-dependent cold response. Serial dilution spot assays were performed on (A) lysogeny broth (LB)-based or (B) tryptic soy broth (TSB)-based agar plates. Exponentially growing cells (OD₆₀₀ = 0.6) in TSB were adjusted to OD₆₀₀ = 0.2 after two washes in 1xPBS. After serial dilutions, 2 µL of each dilution was spotted on the agar plates. The results were recorded after 24 h and 48 h of incubation at 37 °C and 23 °C, respectively. The images are representative of three independent experiments.