Alignment of L. brevis GalP with representative members of the LacS and MelB subfamilies of the GPH family of sugar transporters. The multiple alignment was derived using the TREE program (9). Abbreviations: Lla, Lactococcus lactis; Lbr, Lactobacillus brevis; Llu, Leuconostoc lactis; Sth, Streptococcus thermophilus; Eco, Escherichia coli; GalP, galactose permease; LacP and LacS, lactose permease; MelB, melibiose permease. Complete protein sequences are shown. Completely conserved residues are shaded. Residues conserved only within the LacS subfamily are indicated by ∗. The 12 TMSs are indicated by numbered dashed lines under the alignment.