FIG. 7.

Effect of glucose on [³H]TMG uptake and accumulation in L. brevis. The experiments were conducted as described under Materials and Methods. L. brevis cultures were concentrated in TM buffer (pH 7.0) to a cell density of 2 mg/ml. [³H]TMG accumulation was measured at 30°C. In one set of experiments (●), 20 mM arginine was added to energize the cells, [³H]TMG (100 μM) was added at time −15 min, and 20 mM glucose was added at 0 min to induce [³H]TMG expulsion. In a separate set of experiments (■), glucose (20 mM, added at −1 min) was used as the sole energy source. [³H]TMG was added at 0 min.