β-Catenin activation causes hepatic tumors. (A) Schematic illustration of Cre-adenovirus–induced β-catenin active mutation in mouse liver. (B) Survival of WT mice (n = 23) and β-cateninlox(ex3)/+ mice (n = 27) 50 d post Cre-adenovirus tail vein injection. (C) Representative liver pictures of 13-mo-old mice. (D) Tumor incidence (at least one visible tumor nodule on the surface of liver) of 13-mo-old β-cateninlox(ex3)/+ mice (n = 26). (E) The maximum liver tumor size (Left), tumor number (Middle), and ratio of liver weight to body weight (Right). WT mice (n = 12), β-cateninlox(ex3)/+ mice (n = 26). Data are shown as mean ± SD. (F) Immunoblotting of mouse liver tissues. (G) Representative liver H&E, reticular fiber, and CD34 stainings of β-cateninlox(ex3)/+ mice. (H) Immunohistochemistry stainings of mouse liver tissues. (Scale bar: 100 μm.) ***P < 0.001.