Skip to main content
. 2022 Sep 19;119(39):e2210978119. doi: 10.1073/pnas.2210978119

Fig. 4.

Fig. 4.

CC and WQQ domains of GRP23 and the C terminus of NUWA have similar function and probably interact with MORF1 and MORF8. (A) Detection of the interaction between GRP23 and NUWA in Arabidopsis protoplasts. (Scale bars, 5 μm.) (B) Schematic illustrations of GRP23 and NUWA. (C) Chimeric proteins between GRP23 and NUWA were created to complement the grp23 or nuwa mutant. (D) Morphology of the wild type and grp23 or nuwa mutant complemented by chimeric proteins. The seeds were geminated on MS media, and 1-wk-old seedlings were transferred to soil and grown in the greenhouse under long-day conditions (16 h light/8 h darkness). Images of 4-wk-old plants of the wild type and the complemented mutants were taken. (E) The interactions between the truncations of GRP23 with MORF1 and MORF8. −TL, −TLHA, and −TLHA+X-α-Gal indicate SD/−Trp−Leu, SD/−Trp−Leu−His−Ade, and SD/−Trp−Leu−His−Ade containing X-α-Gal dropout plates, respectively. Images were taken after 3 d of incubation at 30 °C.