FIG. 2.

Stability of ResD∼P and ResE∼P. (A) ResD∼P (3 μM) was purified free from ResE∼P and unbound ATP according to the experimental procedures. The 20-μl aliquots of ResD∼P were taken at 1, 2, 5, 10, 15, 20, 30, 45, 50, and 60 min, as indicated, denatured by addition of 6× SDS sample buffer, and subjected to SDS-PAGE. The dried gel was exposed to a PhosphorImager. (B) Stability of phosphorylated ∗ResE∼P. ∗ResE∼P (3 μM), free from unbound ATP, was treated as described above for ResD∼P. (C) Quantitation of results from panels A and B by PhosphorImaging. The activities of ∗ResE∼P (⧫) and ResD∼P (■) are shown.