FIG. 6.

ResD∼P plus the RNAP core enzyme with ς^A is sufficient for in vitro expression from the upstream ctaA (Pv) but not for expression of the downstream (Ps) promoter. (A) Lane 1, core RNAP plus ς^A; lane 2; core RNAP plus ς^A and ResD; lane 3, core RNAP plus ς^A and ResD∼P; lane 4, core RNAP alone; lane 5, ς^A alone; lane 6, T4 stage RNAP plus ResD∼P. (B) Lane 1, no RNAP; lane 2, T₀ sigE mutant RNAP; lane 3, T₀ sigE mutant RNAP plus ResD; lane 4, T₀ sigE mutant RNAP plus ResD∼P; lane 5, T₄ sigE mutant RNAP; lane 6, T₄ sigE mutant RNAP plus ResD; lane 7, T₄ sigE mutant RNAP plus ResD∼P; lane 8, T₄ sigE mutant RNAP plus ResD∼P and ς^A; lane 9, T₄-stage RNAP plus ResD∼P. The DNA template, reaction mixture, and sample analysis were as described for Fig. 4. WT, wild type; −, absent; +, present; M, 100-nt RNA marker. ResD was added at 100 pMol. ResE was added at 100 pMol.