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. 2022 Aug 27;36(10):2509–2518. doi: 10.1038/s41375-022-01674-2

Fig. 2. Zrsr2 mutation and Tet2 loss expand LT-HSC and impair myelo-erythroid differentiation.

Fig. 2

a Flow cytometry analysis of the LSK compartment from 3-months-old WT (n = 21), Zrsr2m/m (n = 18), Tet2−/− (n = 9), and Zrsr2m/mTet2−/− mice (n = 18). Representative dot plots are shown in Supplementary Fig. 2. b Flow cytometry analysis of myelo-erythroid progenitors from 3-months-old WT (n = 21), Zrsr2m/m (n = 18), Tet2−/− (n = 9), and Zrsr2m/mTet2−/− (n = 18) mice. Representative dot plots are shown in Supplementary Fig. 2. c Left and middle panel: GM-CFU and BFU-E forming capacity of unfractionated BM from 3-month-old WT (n = 18), Zrsr2m/m (n = 12), Tet2−/− (n = 10), and Zrsr2m/m Tet2−/− (n = 17) mice. Right panel: Serial replating capacity of unfractionated BM from 3-months-old WT (n = 3), Zrsr2m/m (n = 3), Tet2−/− (n = 3), and Zrsr2m/m Tet2−/− (n = 6) mice cultured under myeloid conditions. Data represent mean ± SEM.