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. 2022 Aug 27;36(10):2509–2518. doi: 10.1038/s41375-022-01674-2

Fig. 3. Concomitant Zrsr2 mutation and Tet2 loss result in PB cytopenias and multilineage dysplasia.

Fig. 3

a Hematological parameters from 3-month-old WT (n = 28), Zrsr2m/m (n = 21), Tet2−/− (n = 12), and Zrsr2m/mTet2−/− mice (n = 26). WBC: white blood cells, RBC: red blood cells, HGB: hemoglobin, PLT: platelets. Boxes show mice presenting cytopenias. b Peripheral blood smears and bone marrow cytospins from 3-months-old WT and Zrsr2m/mTet2−/− mice stained with May-Grünwald Giemsa. Normal RBC from WT (Ai) and dysplastic RBC from Zrsr2m/mTet2−/− mice (Aii) showing RBC anisocytosis, polychromasia, and presence of nucleated erythrocytes. Normal platelets from WT (Bi) and dysplastic platelets from Zrsr2m/mTet2−/− mice (Bii) showing anisocytosis and giant size. Normal neutrophil from WT (Ci), hypersegmented neutrophil (Cii) and hyposegmented neutrophils with pseudo Pelger-Huët anomalies (Di–Dii) from Zrsr2m/mTet2−/− mice. Nuclear fragmentation and karyorrhexis of granulocytes (Ei-Eii). Erythrophagocytosis (Fi) and binucleated erythroid precursors (Fii) in BM of Zrsr2m/mTet2−/− mice. Normal myeloid precursors from WT (Gi) and dysplastic myeloid precursors with cytoplasm vacuolization from Zrsr2m/mTet2−/− mice (Gii). Normal megakaryocyte in WT (Hi) and giant megakaryocyte in Zrsr2m/mTet2−/− mice (Hii). Dysplastic features are indicated with arrows.