Skip to main content
. 2022 Sep 29;12:16305. doi: 10.1038/s41598-022-20177-9

Figure 1.

Figure 1

Identification of ERα in human conjunctival goblet cells cultured from males and females. RNA was isolated from cultured goblet cells and RT-PCR performed using primers for human Erα. A single band was detected at around 100 bp in samples from 4 out of 5 male (M) and 3 out of 3 females (F) (A). Protein samples were collected from cell pellets and Western Blot analysis was performed using antibody against ERα. A major band at 50 kDa was detected in males, while a major band at 75 kDa and a minor band between 75 and 50 kDa were detected in females (B). Immunofluorescence microscopy was performed on cultured goblet cells using antibodies to ERα (C and D). (C) indicate immunofluorescence to ERα (red) in male cells; (D) indicate immunofluorescence to ERα (red) in female cells; an overlay of anti-ERα, HPA-1 (green, indicates the secretory granules of goblet cells), and DAPI (blue, indicates cell nuclei) is shown next to the single channel image of ERα. Magnification was × 200 in the upper panels; × 1000 in the lower panels. Full gels and blots are included in “Supplementary information”. Original blots/gels are presented in Supplementary Figs. 5 and 6.