Figure 4.
Human conjunctival goblet cells cultured from males and females respond to a neurotransmitter, inflammatory mediator, and allergic mediator. Cultured goblet cells from both sexes were stimulated with carbachol(Cch) 10–5–10−3 M (A and B) or LTB4 10–11–10−8 M (C and D) or histamine(His) 10–8–10−5 M (E and F). [Ca2+]i measured by fura2 assay. The average [Ca2+]i level over time was shown in (A, C and E); Change in peak [Ca2+]i was calculated and shown in (B, D and F). Number of individuals is indicated on each graph. Data are mean ± SEM; the number of individuals is marked in each graph. Arrow indicates the addition of stimuli. Black lines and solid black bars indicate data from male cells, grey lines and slash-patterned bars indicate data from female cells. *Significance difference from basal. #Significance difference between male and female values.