Figure 6.

Overnight treatment with sex hormones inhibits the Ca²⁺ response from human conjunctival goblet cells cultured from male and female cells to the inflammatory mediator LTB₄. Cultured goblet cells from male and female conjunctiva were treated with DHT, E1 or E2 (10⁻⁷ M) or vehicle overnight prior to addition LTB₄ (10⁻⁸ M). [Ca²⁺]_i was measured by fura2 assay. The average [Ca²⁺]_i level over time was shown in (A, C and E); Change in peak [Ca²⁺]_i was calculated and shown in (B, D and F). Data are mean ± SEM. Number of individuals for each experiment are marked in graphs. Black lines and solid bars indicate data from male cells. Grey lines and slash-patterned bars indicate data from female cells. *Significance difference from basal. ^#Significance difference between males and females.