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. 2022 Sep 29;13:5730. doi: 10.1038/s41467-022-33326-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Irf1^–/– and control mice were infected with C. rodentium. a Total RNA of distal colonic tissue (8 dpi) was analyzed by specific qRT-PCR. (Il17,Ifng: Irf1^+/–: n = 8, Irf1^–/–: n = 7; Il22: Irf1^+/–: n = 12, Irf1^-/–: n = 9). b Serum-concentrations of IL-17A, IFN-γ and IL-22 were measured by specific ELISAs. (IL-17: Irf1^+/–: n = 10, Irf1^-/–: n = 11; IFN-γ: Irf1^+/–: n = 10, Irf1^–/–: n = 10; IL-22: Irf1^+/–: n = 12, Irf1^–/–: n = 8). c, d, e Irf1^ΔTie2 and littermate control mice were infected with C. rodentium. c Total RNA of distal colonic tissue (8 dpi) was analyzed by specific qRT-PCR. (Il17: Irf1^fl/fl: n = 14, Irf1^ΔTie2: n = 12;,Ifng: Irf1^fl/fl: n = 13, Irf1^ΔTie2: n = 12; Il22 Irf1^fl/fl: n = 12, Irf1^ΔTie2: n = 13). d Serum-concentrations of IL-17A, IFN-γ and IL-22 were measured by specific ELISAs. (IL-17: n = 14/group, IFN-γ: Irf1^fl/fl: n = 13, Irf1^ΔTie2: n = 12, IL-22: Irf1^fl/fl: n = 12, Irf1^ΔTie2: n = 14). e Serum-concentrations of IL-17A, IFN-γ and IL-22 were measured by specific ELISAs. (IL-17, IL-22: Irf1^fl/+ Tie2cre⁺: n = 5/group, Irf1^fl/fl Tie2cre⁺: n = 6, IFN-γ: Irf1^fl/+ Tie2cre⁺: n = 5, Irf1^fl/fl Tie2cre⁺: n = 5). f Chimeric mice were generated by reconstitution of irradiated C57BL/6 mice with Irf1^+/+ or Irf1^–/– bone marrow. 8 weeks later, chimeras were infected with C. rodentium and serum-concentrations of IL-17A, IFN-γ and IL-22 were measured by specific ELISAs (9 dpi). (IL-17, IFN-γ: Irf1^+/+: n = 8, Irf1^–/–: n = 3; IL-22: Irf1^+/+: n = 10, Irf1^–/–: n = 4). Data is expressed as mean ± SEM. Exact p values defined by two-tailed Mann–Whitney U test are provided in the plots. Source data are provided as a Source data file.