Fig. 5. Irf1 expression regulates cytokine responses of intestinal innate lymphoid cells.

a–d Irf1^–/– and Irf1^+/– control or Irf1^ΔTie2 and Irf1^fl/fl control mice were infected with C. rodentium. LPMCs were isolated at 8 dpi and analyzed by flow cytometry. Graphs show relative abundances and frequencies of Tbet⁺ ILCs (lin^-Thy1.2⁺Tbet⁺), IFN-γ producing Tbet⁺ ILCs (lin^-Thy1.2⁺Tbet⁺IFN-γ⁺), ILC1s (lin^-Thy1.2⁺Tbet⁺EOMES^-NK1.1⁺Nkp46⁺) as well as relative abundances and frequencies of RORγt⁺ ILC3s (lin^-Thy1.2⁺RORγt⁺) and ILC3s expressing IL-22 (lin^-Thy1.2⁺RORγt⁺IL-22⁺). a n = 5/group; (b) n = 3/group; (c) Irf1^+/–: n = 8, Irf1^+/–: n = 9. d Irf1^fl/fl: n = 9, Irf1^ΔTie2: n = 8. e Comparison of intestinal ILC2 frequencies in Irf1^–/– and Irf1^+/– control mice in the steady state or in the context of C. rodentium infection (CR); n = 3/group. Data is expressed as mean ± SEM. Exact p values defined by two-tailed Mann–Whitney U test are provided in the plots. Source data are provided as a Source data file.