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. 2022 Sep 29;13:5733. doi: 10.1038/s41467-022-33367-w

Fig. 2. Obesity, hyperphagia, and increased insulin secretion upon deletion of mir-7 in Sim1 neurons.

Fig. 2

a Experimental strategy to assess the functions of miR-7 in hypothalamic neuron populations. b–e Body weight of chow-fed male (n = 15 and 13 animals) (b), chow-fed female (n = 8 and 11 animals) (c), HFD-fed male (n = 10 and 19 animals) (d), and HFD-fed female (n = 18 and 13 animals) (e) mir-7fl/fl and Sim1-cre;mir-7fl/fl mice. f Representative photograph of HFD-fed female mir-7fl/fl and Sim1-cre;mir-7fl/fl mice. g–h Male and female body weight in 20 week-old chow-fed (g) and HFD-fed (h) mir-7fl/fl and Sim1-cre;mir-7fl/fl mice, using data shown in Fig. 2b–e. Dashed line indicates the body weight of mir-7fl/fl males. i–j Lean and fat mass (n = 8 animals) (i) and adipose depot mass (n = 4 animals) (j) of HFD-fed female mir-7fl/fl and Sim1-cre;mir-7fl/fl mice. k–p 72-hour metabolic cage measurements of HFD-fed female mir-7fl/fl and Sim1-cre;mir-7fl/fl mice (n = 7 animals). (k) Cumulative food intake; (l) average food intake per 12-h phase; (m) hourly energy expenditure; (n) average energy expenditure per 12-hour phase; (o) cumulative locomotor activity; (p) average locomotor activity per 12-hour phase. Dark phases are shaded in grey. q–t Random fed blood glucose (n = 8 and 11 animals) (q), blood glucose during an ipGTT (n = 8 and 11 animals) (r), blood glucose during an ipITT (n = 6 and 9 animals) (s), and plasma insulin during an ipGTT (n = 6 animals) (t) in chow-fed female mir-7fl/fl and Sim1-cre;mir-7fl/fl mice. u–x Random fed blood glucose (n = 18 and 13 animals) (u), blood glucose during an ipGTT (n = 16 and 12 animals) (v), blood glucose during an ipITT (n = 16 and 12 animals) (w), and plasma insulin during an ipGTT (n = 7 animals) (x) in HFD-fed female mir-7fl/fl and Sim1-cre;mir-7fl/fl mice. Data are presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001; no asterisk indicates P > 0.05; 2-way repeated measures ANOVA with Sidak’s multiple comparisons test (b, c, d, e, g, h, l, n, p, r, s, t, v, w, x), two-tailed t test i, j, or 2-way repeated measures ANOVA (k, m, o, q, u). Source data are provided as a Source Data file.