Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Sep 29;13:5733. doi: 10.1038/s41467-022-33367-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a–b Representative image (scale bar: 250 μm) (a) and quantification (n = 5 and 4 animals) (b) of tdTomato-positive cells within the PVN of Sim1-cre;LSL-tdTomato and Sim1-cre;LSL-tdTomato;mir-7^fl/fl mice. Magenta, tdTomato; blue, Hoechst. c Schematic of sample preparation for RNA sequencing of Sim1 neurons from Sim1-cre;LSL-tdTomato (“WT”) and Sim1-cre;LSL-tdTomato;mir-7^fl/fl (“KO”) mice. d–e miR-7a (d) and miR-7b (e) expression in tdTomato-negative and tdTomato-positive FACS-sorted cells from hypothalamus of Sim1-cre;LSL-tdTomato and Sim1-cre;LSL-tdTomato;mir-7^fl/fl mice (n = 4 samples, with 4 mice pooled per sample). Expression is relative to tdT-negative cells from Sim1-cre;LSL-tdTomato mice. f –log10(q-value) and log2(fold change) of all expressed genes in Sim1-cre;LSL-tdTomato;mir-7^fl/fl versus Sim1-cre;LSL-tdTomato RNA sequencing data. Significantly regulated genes (q < 0.05) are indicated in red. g log2(fold change) of key neuropeptide-encoding genes (left panel), and Mc4r, Nos1, Sim1, and Cyrano (right panel) in Sim1-cre;LSL-tdTomato;mir-7^fl/fl versus Sim1-cre;LSL-tdTomato RNA sequencing data (n = 4 samples, with 4 mice pooled per sample). h –log10(p-value) and activation z-scores of top upstream regulators identified by QIAGEN Ingenuity Pathway Analysis of Sim1-cre;LSL-tdTomato;mir-7^fl/fl versus Sim1-cre;LSL-tdTomato RNA sequencing data. Numbers in the bars indicate the number of genes contributing to the prediction. i –log10(p-value) and odds ratio of top transcription factors identified by Enrichr “ENCODE and ChEA Consensus Transcription Factors from ChIP-X” of downregulated genes in Sim1-cre;LSL-tdTomato;mir-7^fl/fl versus Sim1-cre;LSL-tdTomato RNA sequencing data. Numbers in the bars indicate the number of genes contributing to the prediction. j–k Representative immunofluorescence (scale bar: 250 μm) (j) and quantification (n = 5 and 4 animals) (k) of p-CREB intensity in p-CREB+ /tdTomato+ cells and p-CREB+ /tdTomato– cells in the PVN of Sim1-cre;LSL-tdTomato and Sim1-cre;LSL-tdTomato;mir-7^fl/fl mice. Green, p-CREB; magenta, tdTomato; blue, Hoechst. Data are presented as mean ± SD, where error bars are present. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; no asterisk indicates P > 0.05; two-tailed t test (b), 2-way ANOVA with Sidak’s multiple comparisons test (d, e, k), or Fisher’s exact test (h, i). *q < 0.05; **q < 0.01; ***q < 0.001; ****q < 0.0001; quasi-likelihood (QL) differential expression test (f, g). Source data are provided as a Source Data file.