Figure 1. Low-Level Engraftment of Adult Mobilized Blood HSPCs in Immune-Deficient Mice.

(A) Frozen mobilized blood from a male adult rhesus was processed via MACS beads to isolate CD34⁺ cells for injection. Post-enrichment purity in this example was 91.9%. 1×10⁵–1×10⁶ cells were IV injected per mouse for all experiments. (B) Representative flow plots of NHP mice (top) showing overall NHP-CD45 vs. Mouse-CD45 engraftment and CD20⁺ B cell and CD3⁺ T cell frequency of the NHP-CD45⁺ cells, compared to human cord blood CD34⁺ injected controls (bottom). 1×10⁵ CD34⁺ cells were injected, and 55cGY-irradiated NOG-EXL strain was used for both sets of mice. NOG-EXL mice harboring human IL3 and GM-CSF transgenes were injected with 1×10⁶ NHP-CD34⁺ cells per animal and monitored for chimerism (representative animal shown in C) over time in multiple animals (D). Results in D are shown at 8–11 weeks post-injection, compiled from n=2 separate experiments. Value of ≤0.1 and/or <4 positive events were considered background and listed as 0% engraftment.