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. Author manuscript; available in PMC: 2023 Oct 1.
Published in final edited form as: J Leukoc Biol. 2022 Mar 30;112(4):759–769. doi: 10.1002/JLB.5TA0921-481RR

Figure 5. Hematopoietic Engraftment in NHP Mouse Bone Marrow and Secondary Transplantation.

Figure 5.

(A) Bone marrow (BM) was harvested from NHP mouse femurs and analyzed for hematopoietic stem and progenitor markers (lineage negative CD38(lo)CD34+ and CD38(lo)CD34+CD45RA-CD90+ HSPC subsets, values given as a percentage of NHP-CD45+ cells). (B) Total BM cells were harvested from NHP mouse femurs and transplanted via IV injection into secondary, naïve NSG-SGM3 mice, which have human IL3 and GM-CSF transgenes, similar to the NOG-EXL, but also harbor a human SCF transgene. 2×106 total cells injected per animal, n=8, 5 males and 3 females. NOG-EXL mice were also injected (2×106 total cells per animal) and monitored for engraftment over the course of 7 weeks. N=4 male mice, two mice died between weeks 4 and 5.